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The atopic dermatitis is characterized by disruption of skin barrier and colonization of S.aureus. However, the mutual impact of damaged skin barrier and increased S.aureus has not been fully elucidated. Here we used the SKH-1 hairless mice to establish acute barrier dysfunction model and evaluated the mutual impact of damaged skin barrier function and colonization of S.aureus. Skin barrier was destroyed by repeat tape-stripping. Then 36 SKH-1 mice were divided into 6 groups, namely the control group, barrier impaired group, S. aureus inoculated group, barrier impaired +S. aureus inoculated group, “wrapping group” (referred to barrier impaired +wrapping+S. aureus inoculation) and “moisturizing group” (referred to barrier impaired + moisturizer + S. aureus). S. aureus on the skin were collected after 24 hours for verification and quantify. The physiological parameters related to skin barrier were detected before and immediately after impairing of barrier function, after wrapping/moisturizing, 4 hours and 24 hours after inoculation of S. aureus. In this study, we found that There was a little S. aureus grown on intact skin in only inoculation group, while the number of S. aureus in impaired barrier group increased dramatically (P＜0.05); the colonization of S. aureus in both wrapping and moisturizing group decreased obviously compared with the barrier impaired group (P ＜0.05), Fig1, which mean that restoring the barrier function could greatly reduced the colonization of S. aureus. On the other hand the colonization of S. aureus on impaired barrier could inhibit the recovery of skin barrier function, with higher pH and TEWL compared with other groups in similar time points(P＜0.05). The wrapping and moisturizing may eliminate the suppression of barrier function recovery caused by inoculation of S. aureus, Fig 2.
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